We narrowed to 15,981 results for: LEA

1. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti GW V5 Eco/Dam mse EMD

   Plasmid

   #182675

   Purpose

   Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

   Depositor

   Bas van Steensel

   Article

   Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Promoter

   HSP

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-hCYP26A1-FL- luciferase

   Plasmid

   #135566

   Purpose

   Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

   Insert

   Full-length promoter of human retinoic acid receptor-beta gene
   

   Promoter

   hHuman CYP26A1 gene promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

   Plasmid

   #208964

   Purpose

   Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGT6471

   Plasmid

   #219428

   Purpose

   Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
   

   Use

   Moclo compatible level 0 module

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3_T7-mScarlet3-KRas6R

   Plasmid

   #225930

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-KRas6R
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 10_T7-SP-CD8tm-mScarlet3

   Plasmid

   #225937

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   SP-CD8tm-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetOminiCMV-Sox2

   Plasmid

   #136612

   Purpose

   Dox-inducible lentiviral vector expressing mouse Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_eA3A T31A-dCas9

   Plasmid

   #163537

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   dCas9 (D10A, H840A)eA3A T31A

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDR366 RNF2

    Plasmid

    #47509

    Purpose

    human gRNA expression vector targeting RNF2

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-RNF2 (RNF2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Available Since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAPDH::H2B-mScarlet

    Plasmid

    #186764

    Purpose

    A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    H2B-mScarlet
    

    Use

    Overexpression

    Tags

    H2B

    Promoter

    GAPDH

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CD81-(TEV)-FKBP-Myc

    Plasmid

    #177908

    Purpose

    human CD81-TEV cleavage site-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Human, Synthetic)
    

    Tags

    FKBP, Myc tag, and TEV protease cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC446 - pAAV EF1a hPREP(S554A)

    Plasmid

    #59968

    Purpose

    An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S554A

    Promoter

    EF1a

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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