We narrowed to 15,824 results for: LEA

1. pCMV_eA3A T31A-dCas9

   Plasmid

   #163537

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   dCas9 (D10A, H840A)eA3A T31A

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAPDH::H2B-mScarlet

   Plasmid

   #186764

   Purpose

   A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   H2B-mScarlet
   

   Use

   Overexpression

   Tags

   H2B

   Expression

   Mutation

   Promoter

   GAPDH

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-cMyc

   Plasmid

   #136614

   Purpose

   Dox-inducible lentiviral vector expressing mouse cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   cMyc (Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDR366 RNF2

   Plasmid

   #47509

   Purpose

   human gRNA expression vector targeting RNF2

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-RNF2 (RNF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENeGFP PCNAL2 pc653

   Plasmid

   #167564

   Purpose

   The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

   Depositor

   Cristina Cardoso

   Article

   Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-V5-Lem2

   Plasmid

   #120245

   Purpose

   Expresses V5-tagged Lem2 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Lem2 (Lemd2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEmpty

   Plasmid

   #214051

   Purpose

   Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CD81-(TEV)-FKBP-Myc

   Plasmid

   #177908

   Purpose

   human CD81-TEV cleavage site-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Synthetic, Human)
   

   Use

   Tags

   FKBP, Myc tag, and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-G1-ATP8-Myc-FLAG (G418)

   Plasmid

   #86850

   Purpose

   mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Use

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pClneoMyc human MST2

    Plasmid

    #37022

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX_317-C7orf26v1

    Plasmid

    #178537

    Purpose

    Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Insert

    C7orf26v1 (INTS15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid

    #171137

    Purpose

    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 221 to alanine, tryptophan 453…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid

    #129394

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV and Cre/Lox

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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