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pCS2-XTbx5-EnR-GR

Plasmid

#117250

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5-EnR-GR (tbx5-b Frog)

Use

Xenopus expression

Mutation

chimeric protein with human glucocorticoid recep…

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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P23_cymR_Ptec_mCherry

Plasmid

#248113

Purpose

Cumate switch driving mCherry expression

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al J Control Release. 2025 Sep 24;387:11426

Inserts

mCherry red fluorescent protein
CymR repressor

Expression

Bacterial

Promoter

P23 and Ptec flanked by cuO operators

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT_superDn29-P2A-GFP

Plasmid

#247162

Purpose

In vitro transcription of human codon optimized engineered superDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(WT)

Plasmid

#245191

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

wild-type

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 4-1BB-eGFP]

Plasmid

#218342

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 4-1BB]

Plasmid

#218328

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCH68 (dAsCas12a-3xKRAB piggyBac)

Plasmid

#217334

Purpose

piggyBac expression of dAsCas12a-3xKRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

dAsCas12a

Use

CRISPR

Tags

nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

Expression

Mammalian

Mutation

E993A

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-NUDT18-C-FLAG-Puro

Plasmid

#195181

Purpose

Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.

Depositor

Stephanie Roessler

Insert

NUDT18 (NUDT18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

minimal CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV298

Plasmid

#204957

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
pyrG

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD59-T2A-GFP

Plasmid

#205451

Purpose

lentiviral plasmid for expression of mouse CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD59 (Cd59a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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CamKIIa:smFP-TVA-RGV

Plasmid

#202594

Purpose

Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

Depositor

Oleksandr Shcheglovitov

Article

Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

Inserts

smFP
TVA
RGV

Use

Lentiviral

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA1

Plasmid

#201590

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-BeYDV-LS-L-11

Plasmid

#197717

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV LSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

Plasmid

#194642

Purpose

Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

CREB (Creb1 Rat)

Use

AAV

Tags

mEGFP

Mutation

S133A

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM2-rABE-Flag

Plasmid

#191386

Purpose

Doxycycline inducible expression of PUM2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM2-rABE (PUM2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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eDrTrkA

Plasmid

#183996

Purpose

Optically controlled eDrTrkA expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkA (NTRK1 Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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