We narrowed to 32,755 results for: eng

1. pGEX GAC F7

   Plasmid

   #227829

   Purpose

   To express in E. coli GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   GAC isoform of F7 (GLS Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P137, L139, E140, L142, Y145, G150, Q151, E152, K…

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHM1 KGA-33A

   Plasmid

   #227836

   Purpose

   Used for microinjection of nematodes for expression of KGA-33A, GLS with 33 residues mutated to alanine at the interface for interacting with PDZD8

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   KGA isoform (GLS Human)
   

   Tags

   GFP

   Expression

   Worm

   Mutation

   P137, L139, E140, L142, Y145, G150, Q151, E152, K…

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX KGA F7

   Plasmid

   #227822

   Purpose

   To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   KGA isoform of F7 (GLS Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P137, L139, E140, L142, Y145, G150, Q151, E152, K…

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBOBI C-MYC KGA F7

   Plasmid

   #227821

   Purpose

   To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   KGA isoform of F7 (GLS Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   P137, L139, E140, L142, Y145, G150, Q151, E152, K…

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI

   Plasmid

   #196621

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-BQ.1.1 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, H69-, V70-, G142D, …

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI

   Plasmid

   #196620

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-BJ.1 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

   Plasmid

   #176332

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRUPATH Triple Gai2

   Plasmid

   #196049

   Purpose

   Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai2-RLuc8 (GNAI2 Human)
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRUPATH Triple Ga11

   Plasmid

   #196059

   Purpose

   Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha11-RLuc8 (GNA11 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRUPATH Triple Ga15

    Plasmid

    #196060

    Purpose

    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRUPATH Triple Gagust

    Plasmid

    #196054

    Purpose

    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRUPATH Triple GaZ

    Plasmid

    #196053

    Purpose

    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid

    #198465

    Purpose

    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-XBB.1.5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid

    #60224

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Promoter

    EFS, No promoter, and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Expression

    Mammalian

    Available Since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DH10B E. coli aptI/gidB::Landing Pad

    Bacterial Strain

    #83036

    Purpose

    For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

    Depositor

    Cammie Lesser

    Article

    Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

    Bacterial Resistance

    Tetracycline

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

17. PermaPhos Kit

    Plasmid Kit

    #1000000226

    Purpose

    This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

    Depositor

    Ryan Mehl

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

18. NT-CRISPR plasmid collection

    Plasmid Kit

    #1000000189

    Purpose

    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.

    Depositor

    Anke Becker

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

19. TALE Toolbox

    Plasmid Kit

    #1000000019

    Purpose

    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.

    Depositor

    Feng Zhang

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pVSVdG-4BFP2

    Plasmid

    #64101

    Purpose

    Expresses mTagBFP2

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge