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pAGM24951

Plasmid

#169088

Purpose

MoClo level 0 Tomato E8 promoter module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Tomato E8 promoter

Use

Synthetic Biology; Moclo cloning vector

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJH61

Plasmid

#172143

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*); [strong promoter eG, strong RBS SD8] …

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#2

Plasmid

#170838

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#3

Plasmid

#170839

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#1

Plasmid

#170837

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Venus-Actb

Plasmid

#169788

Purpose

An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-YFP-shSCR

Plasmid

#139647

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Publication

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

See Depositor comments

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-Cavin1-GFP

Plasmid

#170859

Purpose

Mammalian expression of His-Cavin1-GFP.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

caveolae associated 1 (Cavin1) (Cavin1 Mouse)

Tags

(His)6 and GFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDHAS

Plasmid

#160920

Purpose

Dihydroxyacetone synthase (DHAS) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFDH1

Plasmid

#160913

Purpose

Formate dehydrogenase (FDH1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

formate dehydrogenase promoter

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFLD1

Plasmid

#160914

Purpose

Formaldehyde dehydrogenase (FLD1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formaldehyde dehydrogenase promoter

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAG500-FKBP-N-frFAST

Plasmid

#160060

Purpose

Expresses FKBP-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FKBP-N-frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAG502-MAP4-frFAST

Plasmid

#160058

Purpose

Expresses MAP4-frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

MAP4-frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pWRKY70::LUC

Plasmid

#141171

Purpose

Luminescent reporter for salicylic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Publication

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtWRKY70 promoter

Use

Luciferase

Expression

Plant

Promoter

AtWRKY70, AT3G56400

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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