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Plasmid#213173PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADORA1 (ADORA1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis WT
Plasmid#213603PurposeExpresses PLA2G15-6xHis in mammalian cellsDepositorAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-SpdCas9
Plasmid#210703PurposeThis plasmid express EFS promoter driven human codon optimized SpdCas9DepositorInsertS. Pyogenes dCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A/H840APromoterEF1a core promoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0mut
Plasmid#208718PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVPromoterhSynAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_g5-HT3.0
Plasmid#208727PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytesDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterGfaABC1DAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2B
Plasmid#202728Purposeexpresses fluorescent protein-tagged enzymeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTagBFP2:PIP4K2B (PIP4K2B Human)
ExpressionMammalianAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCd1-mNGx1
Plasmid#202751Purposeexpresses fluorescent protein-tagged lipid biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLCD1v2(1-170):mNG2 (PLCD1 Human)
ExpressionMammalianAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[TEV]
Plasmid#200122PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
UseRetroviralExpressionMammalianPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-rtTA-Antares
Plasmid#197587PurposeExpresses TET-On reverse transactivator labeled with AntaresDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrtTA-P2A-Antares
TagsAntaresExpressionMammalianPromoterEF-1alphaAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD8
Plasmid#195846PurposeExpresses human FZD8 with an N-terminal HiBiTDepositorAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 KRAB
Plasmid#188504PurposeExpresses FLAG tagged SadCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-lung (UPB2)
Plasmid#180788PurposeUnique Projection Barcode (UPB2)-Lung for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEx-insertion-erm-T1T2
Plasmid#172212PurposeFor genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminatorDepositorPublicationTypeEmpty backboneUseGenetic insertion in prevotella copriAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPIDCB2
Plasmid#169900PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionExpressionMammalianPromoterDox inducibleAvailable sinceDec. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7
Plasmid#175536PurposeExpression of HaloTag7 at the at the Golgi apparatus of mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta4Gal-HaloTag7
ExpressionMammalianAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-TRE-ECC-Biosensor
Plasmid#140833PurposeEncoding the TRE promoter based eccDNA biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRE-ECC Biosensor
UsePiggybac (pb) transposonExpressionMammalianPromoterinverseAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN2B TKIT donor
Plasmid#169431PurposeDonor DNA for TKIT knockin of SEP to NR2B in mouseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only