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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

myr-mTagBFP2-Flag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Promoter

Lac

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC

Plasmid

#148394

Purpose

Insect Expression of DmAGO1-L819Y-siRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmAGO1-L819Y-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC

Plasmid

#148395

Purpose

Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 silent mutations compared to the…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

Plasmid

#148404

Purpose

Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC

Plasmid

#148414

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1318-1605-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_O

Plasmid

#147097

Purpose

Insect Expression of DmPABPC1-siRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmPABPC1-siRNAres (pAbp Fly)

Expression

Insect

Mutation

seven silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N

Plasmid

#147024

Purpose

Mammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L

Plasmid

#146859

Purpose

Insect Expression of DmPABPC1_1-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_1-550-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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