We narrowed to 10,873 results for: kars;

1. pCS2-3HA-Mefv iso1

   Plasmid

   #92105

   Purpose

   Expression in eukaryotic cells

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-TO-HA-SH3BP1 (RNAi-res)

   Plasmid

   #136645

   Purpose

   Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Maria Balda, Karl Matter

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1 (RNAi resistant) (SH3BP1 Human)
   

   Use

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. paGFP-HRas G12V

   Plasmid

   #18694

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   H-Ras G12V (HRAS Human)
   

   Use

   Tags

   photoactivatable GFP

   Expression

   Mammalian

   Mutation

   A206K mutation in paGFP G12V mutation in HRas

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-TetR-NLS-VP16

   Plasmid

   #103834

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-TetR-NLS-VP16 (tetR )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDNA3-Cerulean-FHA1-NES

   Plasmid

   #138202

   Purpose

   Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   Cerulean-FHA1-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRVdG-4RFP

   Plasmid

   #52489

   Purpose

   Expresses TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

   Insert

   TagRFP-T
   

   Use

   Rabies virus

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

   Plasmid

   #136646

   Purpose

   Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

   Depositor

   Maria Balda, Karl Matter

   Article

   Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

   Insert

   SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
   

   Use

   Tags

   HA (MQDLPGNDNSTAG)

   Expression

   Mammalian

   Mutation

   GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDB.His.MBP.3C CARD8 UPA-CARD WT

   Plasmid

   #164021

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGATOR

    Plasmid

    #64102

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ROSA26 endogenous

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPagP

    Plasmid

    #112125

    Purpose

    Bacterial expression of outer membrane protein PagP, wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    PagP (pagP E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-NIrD-Mefv iso1

    Plasmid

    #92108

    Purpose

    inducible expression in eukaryotic cells

    Depositor

    Feng Shao

    Article

    Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

    Insert

    Mefv isoform1 (Mefv Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SLC9A3R1_PDZ_2

    Plasmid

    #103933

    Purpose

    Protein expression and purification of SLC9A3R1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG5-HA-H-Ras_wt

    Plasmid

    #39509

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Eukaryotic

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. NLS-tdPCP-GFP-VPR

    Plasmid

    #183936

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP eIF2beta

    Plasmid

    #45902

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-PKAsub-YPet-NES

    Plasmid

    #138203

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid

    #183924

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP4-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcold-6xHis-HRV3Csite-DmLoqsPA

    Plasmid

    #41093

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PA (loqs Fly)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

22. MigR1-Flag-TRIB2

    Plasmid

    #131154

    Purpose

    Retroviral expression of flag-tagged mouse TRIB2

    Depositor

    Warren Pear, Karen Keeshan

    Article

    Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

    Insert

    Trib2 (Trib2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUE1-TSP-ORF68 K395A/K396A

    Plasmid

    #162656

    Purpose

    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDESTmycEIF2C1

    Plasmid

    #19871

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    eukaryotic translation initiation factor 2C, 1 (AGO1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRVdG-4FLPo

    Plasmid

    #122050

    Purpose

    Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSG5-Myr-FLAG-p110β-DM

    Plasmid

    #55719

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 beta
    

    Use

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    S205D, K224A

    Promoter

    T7

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pcold-6xHis-HRV3Csite-DmLoqsPD

    Plasmid

    #41095

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PD (loqs Fly)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pSG5-H-Ras_V12

    Plasmid

    #39510

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Eukaryotic

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only