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pGGB-V5-TurboID-MUTT2A-C

Plasmid

#218897

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-MUTT2A

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB-V5-TurboID-T2A-C

Plasmid

#218896

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-T2A

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB-Kozak-ATG-C

Plasmid

#218895

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

kozak-ATG

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-W-ccdB-Z

Plasmid

#218893

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-W-A-ccdB-G-X

Plasmid

#218891

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-W-ccdB-Z

Plasmid

#218890

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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MsmegA _pET29b

Plasmid

#215411

Purpose

Expression construct for MsmegA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MsmegA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR-hCD2

Plasmid

#135392

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

gRNA_hIRF1 promoter #12

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV013

Plasmid

#214739

Purpose

Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRV∆L-5tTA

Plasmid

#182966

Purpose

The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

tTA

Use

Rabies

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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