We narrowed to 6,862 results for: GFP expression plasmids

1. pEG302 5aa SunTag VP64 g4+g17 (FWA)

   Plasmid

   #119672

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]

   Plasmid

   #229393

   Purpose

   XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT (PGK) 12xHRE-NanoLuc

   Plasmid

   #208575

   Purpose

   hypoxic response element driven NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   NanoLuciferase
   d2eGFP
   12xHRE
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRMTK-clo39

   Plasmid

   #204014

   Purpose

   Cloning plasmid containing the sfGFP insert with a chloramphenicol resistance marker

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   super folder green fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBRC169-DXMT

   Plasmid

   #79822

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

   Depositor

   Masamitsu Wada, Yutaka Kodama

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaDXMT1
   

   Use

   Reporter plasmid

   Tags

   RC169/DsREDmonomer (169–225)

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter and CaMV35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBRN168-DXMT

   Plasmid

   #79821

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

   Depositor

   Masamitsu Wada, Yutaka Kodama

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaDXMT1
   

   Use

   Reporter plasmid

   Tags

   RN168/DsREDmonomer (1–168)

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter and CaMV35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRC169-MXMT

   Plasmid

   #79820

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Masamitsu Wada, Yutaka Kodama

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   RC169/DsREDmonomer (169–225)

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter and CaMV35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-S5E2-Gq-P2A-dTomato

   Plasmid

   #135635

   Purpose

   AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV PHP.eB, AAV1, and AAV9

   Insert

   Gq-P2A-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL001_Hec1-ms_IgG_HC

   Plasmid

   #193618

   Purpose

   Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
   

   Use

   Tags

   His6 and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDL002_Hec1-ms_IgG_LC

    Plasmid

    #193619

    Purpose

    Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Hec1 light chain (Hec1-ms_IgG_LC)
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. FHp5pUP95C3,5SGW (E5)

    Plasmid

    #74035

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Oliver Schluter

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

12. FUP95DSKGW (C8)

    Plasmid

    #74024

    Purpose

    lentiviral expression of Psd95-delta SK-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Oliver Schluter

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    delta SK

    Promoter

    hUbC

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUt-mNF-BACH1

    Plasmid

    #199219

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid

    #225947

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3E S17E S19E

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid

    #225948

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3A S17A S19A

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid

    #225956

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Full-length CKAP4 with additional luminal domain …

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid

    #225944

    Purpose

    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    ATL1 (ATL1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid

    #119554

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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