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Lenti-guide-puro mYwhae -2

Plasmid

#198498

Purpose

lentiviral stable expression of mYwhae gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mYwhae (Ywhae Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mYwhae - 1

Plasmid

#198497

Purpose

lentiviral stable expression of mYwhae gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mYwhae (Ywhae Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197958

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Expression

Plant

Promoter

FRO6

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI046

Plasmid

#170842

Purpose

(pUBQ10::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pUBQ10::SMT2-FLAG::tOCS

Expression

Plant

Promoter

pUBQ10

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-Con/Fon-EYFP-WPRE

Plasmid

#186752

Purpose

AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EYFP

Use

AAV

Promoter

CBA

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTL370

Plasmid

#69880

Purpose

attB flanked genomic Cirl for integration at attP sites.

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.HA.HA.Mm.RAP1.I312R

Plasmid

#184643

Purpose

Retroviral expressions of mouse RAP1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

HA FLAG

Expression

Mammalian

Mutation

I312R

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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1056H

Plasmid

#183135

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056J

Plasmid

#183136

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056K

Plasmid

#183137

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

Plasmid

#105194

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

Plasmid

#105195

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(LC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1(K569A,K571A)-StrepII

Plasmid

#174426

Purpose

Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

K569A, K571A

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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IBMc397

Plasmid

#161955

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

Expression

Bacterial

Promoter

PBAD

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1(K557A,K560A)-StrepII

Plasmid

#174425

Purpose

Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

K557A, K560A

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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