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pEGFPc1 Rab34

Plasmid

#237338

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

Rab34 (RAB34 Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-NPL4 NZF (574-608)

Plasmid

#235653

Purpose

Bacterial expression of human NPL4 NZF (574-608)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

NPL4 NZF (NPLOC4 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 574-608 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF5 (411-444)

Plasmid

#235649

Purpose

Bacterial expression of human CAPN15 ZF5 (411-444)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF5 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 411-444 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF4 (337-369)

Plasmid

#235648

Purpose

Bacterial expression of human CAPN15 ZF4 (337-369)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF4 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 337-369 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF3 (141-173)

Plasmid

#235647

Purpose

Bacterial expression of human CAPN15 ZF3 (141-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 141-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

aac6-aph2-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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EW269 CMV-TO-Gal4-NZF[GS Linkers] (FLP-IN)

Plasmid

#236122

Purpose

Plasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

Gal4-NZF (GS Linkers)

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBX_RPS25_smRuby2_HA

Plasmid

#236090

Purpose

Constitutively encodes RPS25-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting RPS25 in the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS25-smRuby2-HA (RPS25 Human, Synthetic)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_C74_eMagAF_V5_NES

Plasmid

#235636

Purpose

Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

eMagAF-V5-NES

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N256_eMagBF_V5_NES

Plasmid

#235630

Purpose

Encodes ALIBi N-terminal fragment, residues 1-256, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-256

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C233

Plasmid

#235629

Purpose

Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 233-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C141

Plasmid

#235621

Purpose

Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 141-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C222

Plasmid

#235627

Purpose

Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 222-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C196

Plasmid

#235625

Purpose

Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 196-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N232_eMagBF_V5_NES

Plasmid

#235628

Purpose

Encodes ALIBi N-terminal fragment, residues 1-232, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-232

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_MyrPalm_NES_V5_eMagAF_C74

Plasmid

#235639

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

MyrPalm-NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_V5_eMagAF_C74_cMycNLS

Plasmid

#235640

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

V5-eMagAF and cMycNLS

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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