We narrowed to 3,409 results for: guide rna expression plasmid

1. pCCM936

   Plasmid

   #58981

   Purpose

   Expresses avr-14 sgRNA in C.elegans.

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   avr-14 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

   Plasmid

   #115487

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

   Plasmid

   #115490

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302 5aa SunTag VP64 g4+g17 (FWA)

   Plasmid

   #119672

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ADEPT-pTarget-GFP

   Plasmid

   #238041

   Purpose

   Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADEPT-pTarget-RFP

   Plasmid

   #238040

   Purpose

   Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Lux

   Available since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADEPT-pTarget

   Plasmid

   #238033

   Purpose

   Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Promoter

   J23119 (constitutive)

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIA33

   Plasmid

   #120803

   Purpose

   E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Inserts

   deactivated nuclease dCas9, codon-optimized for C. difficile
   single guide RNA targeting red fluorescent protein
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Base-pairing region targets red fluorescent prote…

   Promoter

   Pgdh and Pxyl

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-SpCas9-g3

    Plasmid

    #80786

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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