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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,000 results for: sgRNA

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Showing: 6521 - 6540 of 9000 results

  1. pEPOR1CB0019

    Plasmid
    #117555
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117555-plasmid-map-sequence-id-228154

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  2. pEPOR1CB0018

    Plasmid
    #117554
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Expression
    Plant
    Promoter
    AtU6-26 (pICSL90002)
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117554-plasmid-map-sequence-id-228153

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  9. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  19. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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Showing: 6521 - 6540 of 9000 results