We narrowed to 41,947 results for: Eras
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Plasmid#225599PurposeThe STU backbone vector for maize genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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Tensin-2 Y483E
Plasmid#201794PurposeExpresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
TagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483EAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483F
Plasmid#201795PurposeExpresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
TagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483FAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 Y483A
Plasmid#201796PurposeExpresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backboneDepositorInsertTensin-2 (Tns2 Mouse)
TagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationmutation Y483AAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV cleavable His10ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-PRM-wasabi
Plasmid#195554PurposeTemperature-dependent expression of mWasabi reporter with transcription activation by TcI38DepositorInsertsmWasabi
TcI38
Tags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56
Plasmid#208645PurposeRemove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparation for knock-in of…TagsTwin StrepMutationTwo SNP (rs749555789, rs760194863) were introduce…Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-min5UTR-hRluc-A60
Plasmid#182638PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase,
UseLuciferaseExpressionMammalianAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only