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AAV-CK(1.3)GW

Plasmid

#27227

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMB

Plasmid

#218936

Purpose

Expression of N-terminal FLAG tagged human gasdermin B

Depositor

Christopher LaRock

Publication

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMB (GSDMB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kif 13b (Kif13b Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Publication

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Expression

Bacterial

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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hSgk1_v3 Flag

Plasmid

#86039

Purpose

full length human Sgk1_i3 with Flag tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

Insert

Alternate transcript v3 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v3; S313G

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Publication

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(1004)

Plasmid

#44365

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Publication

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCnCas12f1HS_sgRNA_MS13_empty

Plasmid

#204999

Purpose

CnCas12f1 with MS13 sgRNA site for Human cell genome editing

Depositor

Quanjiang Ji

Publication

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Type

Empty backbone

Use

CRISPR

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcsII-pb6-flag

Plasmid

#86770

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 6 (PSMB6 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

unknown

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Publication

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
C-5545 ∆cosδε

Bacterial strain

#209018

Purpose

Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

Depositor

Alfonso Jaramillo

Publication

Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

Bacterial resistance

Hygromycin

Species

Escherichia coli

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR223-LMP1

Plasmid

#195805

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP1

Depositor

Eric Johannsen

Publication

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP1 (LMP-1 Epstein-Barr virus)

Use

Gateway Cloning

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Hd32-IL-13

Plasmid

#163869

Purpose

Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

Depositor

Jakob Reiser

Publication

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TPMV H glycoprotein/IL-13

Tags

Human IL-13

Expression

Mammalian

Mutation

Amino acids 2 to 33 at N terminus deleted

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1 S449A/T450A/S454A

Plasmid

#19847

Depositor

Ron Prywes

Publication

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Publication

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGST1-yHse1-UIM

Plasmid

#44429

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yHse1-UIM (150-190) (HSE1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-tUIM

Plasmid

#44428

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yVps27 tandem UIM (245-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only