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pCMV_CreNLS_PP7

Plasmid

#232439

Purpose

expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cre–MYC-NLS

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-B1

Plasmid

#90319

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGGY003

Plasmid

#48867

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pXP118

Plasmid

#26825

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP122

Plasmid

#26827

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

mNeonGreen

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PI(WT)-Vav2(DPZ)

Plasmid

#86974

Purpose

PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/C1 (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBW300ParsR-Amp32T

Plasmid

#78652

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-ELP[AV-60]

Plasmid

#67012

Purpose

Expresses malE-ELP[AV-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[AV-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-GLP-1

Plasmid

#66997

Purpose

Expresses malE-GLP-1 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-GLP-1

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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ZE3 Wnt-8

Plasmid

#17044

Depositor

Randall Moon

Article

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8 full length (wnt8a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBTK001

Plasmid

#110573

Purpose

Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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3n1 (572)

Plasmid

#70727

Purpose

BetI/PhlF/SrpR BCD7 3-node v41-1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
betI-ssrA
phlF-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-GFP (PX458)

Plasmid

#129026

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSC-hTDG-PreE2

Plasmid

#52272

Purpose

Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro BRIT1

Plasmid

#16206

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

shBRIT1 (MCPH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
1007 pGEX2T PTEN A4

Plasmid

#10770

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

A4: S380A, T382A, T383A, S385A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only