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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 6561 - 6580 of 14582 results

  7. pYPQ284-RVR

    Plasmid
    #138117
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N563R, K569V and N573R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138117-plasmid-map-sequence-id-272074

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  14. pZF-BCB-PDZ

    Plasmid
    #127478
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-PDZ
    Depositor
    Sebastian Maerkl
    Publication
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    Use
    Cell-free transcription–translation
    Tags
    alpha 1-syntrophin PDZ domain
    Promoter
    T7
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127478-plasmid-map-sequence-id-248851

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  15. IFITM3-N21del-V93-TAG

    Plasmid
    #122480
    Purpose
    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon and deleted amin…
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122480-plasmid-map-sequence-id-237764

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  16. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Publication
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  17. pICSL11023

    Plasmid
    #117542
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S_OMEGA(pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117542-plasmid-map-sequence-id-228061

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Showing: 6561 - 6580 of 14582 results