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pQCXIH-Flag Smad3 AAGY
Plasmid#27027DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationP181A P182APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV14_NOP58
Plasmid#73063Purposeexpression clone for human NOP58 with C-terminal 3xFLAG-tagDepositorInsertNOP58 ribonucleoprotein (NOP58 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-TRPM1 cDNA
Plasmid#29782DepositorInsertTRPM1 (TRPM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta WT GFP
Plasmid#128820PurposeTo visualize Doc2beta WT in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-RNF68
Plasmid#126545PurposeExpresses mCherry-tagged RNF68 in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68
Plasmid#126548PurposeExpresses FLAG-HA-tagged RNF68 in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationPromoterLTRAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKG139
Plasmid#69755PurposeRetroviral expression of CENP-CDepositorInsertCENP-C (CENPC Human)
UseRetroviralTagsmCherry-TEV-SExpressionMammalianMutationL341F, N570D relative to NP_001803.2 (numbering b…PromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
S1PR4-Tango
Plasmid#66499PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertS1PR4 (S1PR4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD9 Flag
Plasmid#74901PurposeExpress COMMD9 in mammalian cellsDepositorInsertCOMMD9 (COMMD9 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha WT GFP
Plasmid#128819PurposeTo visualize Doc2alpha WT in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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GFP-MYO10-BioID
Plasmid#194858PurposeExpress MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.DepositorInsertMYO10 (MYO10 Human)
UseTagsBioID and EGFPExpressionMammalianMutationMyoX contains a Q680R variant compared to the NCB…PromoterCMVAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-204PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1PR4-DuET
Plasmid#213375PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertS1PR4 (S1PR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAPH1Δ536-587
Plasmid#194865PurposeExpress eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.DepositorInsertRAPH1 (RAPH1 Human)
UseTagseGFPExpressionMammalianMutationRAPH1 with amino acids 536-587 deletedPromoterCMVAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only
