We narrowed to 705 results for: Bacillus

1. pBz106_CsoS2_MR

   Plasmid

   #140843

   Purpose

   His-tagged CsoS2 Middle Region (MR), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 Middle Region (MR)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   only amino acids 257-603

   Promoter

   T7

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLz75_CB_operon_CsoS2_N3-N4

   Plasmid

   #140857

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-109 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLz77_CB_operon_CsoS2_N3

   Plasmid

   #140859

   Purpose

   Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-108 and 220-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLz78_CB_operon_CsoS2_delNTD

   Plasmid

   #140860

   Purpose

   Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz76_CB_operon_CsoS2_N4

   Plasmid

   #140858

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-189 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBz109_CsoS2_NTD

   Plasmid

   #140842

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 N-terminal domain (NTD)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 262-869

   Promoter

   T7

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBz110_CsoS2_CTD

   Plasmid

   #140844

   Purpose

   His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 C-terminal domain (CTD)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-594

   Promoter

   T7

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ290-D977A

   Plasmid

   #129674

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   D977A; AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ290-E848A

   Plasmid

   #129675

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Tags

   E848A; AacCas12b is rice codon optimized

   Expression

   Plant

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ292-D977A

    Plasmid

    #129680

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    D977A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ292-E848A

    Plasmid

    #129681

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    E848A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA-dabAB2

    Plasmid

    #133012

    Purpose

    pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFE-dabAB2 C351A

    Plasmid

    #133005

    Purpose

    pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Plasmid

    #218667

    Purpose

    pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoLbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-TetOne-Puro-LbNOX-FLAG

    Plasmid

    #218631

    Purpose

    This plasmid contains LbNOX under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPP6

    Plasmid

    #209968

    Purpose

    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter Dropout (sfGFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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