We narrowed to 698 results for: Bacillus

Showing: 641 - 660 of 698 results

Results per page:

Sort results by:

pCRISPR-PsppA-mCherry

Plasmid

#225428

Purpose

Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

Depositor

Geir Mathiesen

Article

Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

Insert

mCherry, Cas9, SgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PsppA upstream of mCherry and Cas9, P3 upstream o…

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB578

Plasmid

#141095

Purpose

E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Inserts

Cas9 and tracrRNA
repeat-spacer-repeat array

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMZ7

Plasmid

#223180

Purpose

pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

Depositor

Paul Blainey

Article

Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

Type

Empty backbone

Use

Chromosomal integration

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Tags

NLS

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ybbR-HIS-Xyl-DocI

Plasmid

#58711

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Xyl-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Threonin 120 to Cysteine in Xylanase Doma…

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292 (AaCas12b)-D570A-TV

Plasmid

#136380

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292-D570A-SRDX

Plasmid

#129684

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b-SRDX

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz74_Rubisco_CsoS2_Npeptide_fusion

Plasmid

#140840

Purpose

Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS

Tags

CsoS2 N-peptide consensus to CbbL C-terminus and …

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ290-D570A-SRDX

Plasmid

#129682

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b-SRDX

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-dabAB2 C539A

Plasmid

#133008

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

C539A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-dabAB2 H524A

Plasmid

#133007

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

H524A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-dabAB2 D353A

Plasmid

#133006

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

D353A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

Plasmid

#136382

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-VPR

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCM’

Plasmid

#162709

Purpose

pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Mutation

p15A origin replaced with high-copy colE1 origin

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

Plasmid

#176587

Purpose

Bacterial expression of His14-MBP-TEV-AmaTnpB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

His14 tag

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b-dabAB2

Plasmid

#133013

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Promoter

t7

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge