Addgene

We narrowed to 2,387 results for: MEA;

Showing: 641 - 660 of 2387 results

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PX458_ZNF511_2

Plasmid

#104045

Purpose

Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 gRNA (ZNF511 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF511

Plasmid

#104037

Purpose

Donor vector for 3' FLAG tag of human ZNF511

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 homology arms (ZNF511 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_RFXANK

Plasmid

#86296

Purpose

Donor vector for 3' FLAG tag of human RFXANK

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RFXANK homology arms (RFXANK Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RFXANK_2

Plasmid

#86365

Purpose

Encodes gRNA for 3' target of human RFXANK

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against RFXANK (RFXANK Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RFXANK_1

Plasmid

#86364

Purpose

Encodes gRNA for 3' target of human RFXANK

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against RFXANK (RFXANK Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NCoA2_1

Plasmid

#86341

Purpose

Encodes gRNA for 3' target of human NCoA2

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NCoA2 (NCOA2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF644_2

Plasmid

#86306

Purpose

Encodes gRNA for 3' target of human ZNF644

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF644 (ZNF644 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF644_1

Plasmid

#86305

Purpose

Encodes gRNA for 3' target of human ZNF644

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF644 (ZNF644 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

Plasmid

#68276

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4E) mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

RPS2

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

Plasmid

#68275

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4A) mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNB689

Plasmid

#60133

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB756

Plasmid

#60149

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB752

Plasmid

#60155

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

ADE17pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB812

Plasmid

#60160

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

Promoter

RNR1pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMA3228

Plasmid

#46856

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY K81M

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-mTurquoise2-Nuf2

Plasmid

#80762

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Ray Truant, Jeffrey Keillor

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Use

Tags

EYFP and mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-YFP

Plasmid

#22400

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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