We narrowed to 11,725 results for: Vars;

1. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

   Plasmid

   #138524

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   PRC2-binding G-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV10-mFoxP1-ES

   Plasmid

   #35169

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-hPMCA2xb

   Plasmid

   #47751

   Purpose

   mammalian expression of mCherry tagged hPMCA2, xb splice variant

   Depositor

   Emanuel Strehler

   Article

   Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

   Insert

   PMCA2x/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   xb splice variant

   Promoter

   CMV

   Available Since

   Nov. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pTER GAMMA-TUBULIN shRNA Human

   Plasmid

   #87955

   Purpose

   reduced the expression of gamma-tubulin in human cells

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   sh gamma-tubulin (TUBG1 Human)
   

   Tags

   No-tag

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene.…

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1_sh_ex15

   Plasmid

   #35166

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pTE4565

   Plasmid

   #88903

   Purpose

   Expresses inactive/dead, humanized MbCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(D986A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1_sh_ex13

   Plasmid

   #35165

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pTE4567

   Plasmid

   #107557

   Purpose

   Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(dead2)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   E1080A

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-tet-puro-sh-ex16

    Plasmid

    #35167

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pRD439

    Plasmid

    #168465

    Purpose

    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mTurquoise2-H-NSdbd-NLS
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3320

    Plasmid

    #107529

    Purpose

    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hLbCpf1(RVR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-tet-puro-sh-ex16b

    Plasmid

    #35168

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAG426GAL-ccdB-mRuby3

    Plasmid

    #166142

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Tags

    mRuby3

    Expression

    Yeast

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4 Ta-sro1 WWE-PARP

    Plasmid

    #192548

    Purpose

    pENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTln1-F2F3-P229L-pET151

    Plasmid

    #177879

    Purpose

    Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mTln1-F2-P229S-pET151

    Plasmid

    #177871

    Purpose

    Expresses mouse Talin 1 F2-P229S domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229S mutant (Tln1 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTln1-F2-P229L-pET151

    Plasmid

    #177876

    Purpose

    Expresses mouse Talin 1 F2-P229L domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229L mutant (Tln1 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTln1-F2F3-P229S-pET151

    Plasmid

    #177878

    Purpose

    Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge