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Showing: 641 - 660 of 3628 results

  2. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236177
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236177-plasmid-map-sequence-id-471373

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  3. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

    Plasmid
    #236173
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236173-plasmid-map-sequence-id-471240

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  4. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

    Plasmid
    #236172
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236172-plasmid-map-sequence-id-468453

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  5. EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236175
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236175-plasmid-map-sequence-id-468447

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  6. EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)

    Plasmid
    #236147
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to FKBP1A, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236147-plasmid-map-sequence-id-468434

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  7. EW492 CMV-TO-UTRN-14ZF(N61K,H88F, I91E, K120F)-realdeImmLink-NZF(FLP-IN)

    Plasmid
    #236150
    Purpose
    Express mutated UTRN-14 zinc finger array (N62K, H89F, I91E, K121F) attached by a deimmunized linker to the NZF transcriptional activation under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    UTRN-14ZF-deImmLink-NZF
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N61K, H88F, I91E, K120F in UTRN-14ZF
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236150-plasmid-map-sequence-id-468429

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  8. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

    Plasmid
    #236159
    Purpose
    Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    NZF-deImmunLink-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236159-plasmid-map-sequence-id-468430

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  10. EW263 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R(FLP-IN)

    Plasmid
    #236126
    Purpose
    Plasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E488Q, T501A in ADAR2dd
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236126-plasmid-map-sequence-id-468414

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  11. EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)

    Plasmid
    #236146
    Purpose
    Plasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    NZF-deImmunLink-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N2093W, T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236146-plasmid-map-sequence-id-468416

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  12. EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236176
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236176-plasmid-map-sequence-id-468380

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  19. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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Showing: 641 - 660 of 3628 results