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Showing: 641 - 660 of 1232 results

  11. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  16. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  18. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid
    #186310
    Purpose
    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg61-PDGFR
    eGFP
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186310-plasmid-map-sequence-id-366939

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Showing: 641 - 660 of 1232 results