-
Plasmid#198197PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linkerDepositorInsertmVenus-3AA-mTurquoise2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-GFP
Plasmid#13770PurposeCre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertEGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST5552
Plasmid#36255PurposeMycobacteria replicating vector expressing eGFP.DepositorInserteGFP
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModifiedHsp60Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-N1
Plasmid#11909DepositorHas ServiceCloning Grade DNAInsertpPAGFP-N1
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-C1
Plasmid#11910DepositorHas ServiceCloning Grade DNAInsertPAGFP
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-qmTQ2-cAMP
Plasmid#225250Purposemammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
UseTagsExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051…PromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-GFP
Plasmid#13772PurposeFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertEGFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP
Plasmid#214367PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-qmTQ2-ATP
Plasmid#225248Purposemammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercptAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-GFP
Plasmid#15949DepositorInsertGreen Fluorescent Protein
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…PromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-EGFP
Plasmid#165040PurposeExpression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)DepositorInsertEGFP
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSfinx-mYFP
Plasmid#216206PurposeA simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamianaDepositorInsertmonomeric yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only