Addgene

We narrowed to 12,812 results for: sequence

Showing: 641 - 660 of 12812 results

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pCTcon2-dHUH

Plasmid

#235187

Purpose

To display dHUH on yeast surface

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

dHUH

Use

Tags

HA, aga2p, and myc

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2S-C

Plasmid

#225967

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of serine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2S-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2N-C

Plasmid

#225968

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of asparagine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2N-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2D-C

Plasmid

#225969

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2D-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2E-C

Plasmid

#225970

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2E-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2A-C

Plasmid

#225965

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2A-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2H-C

Plasmid

#225966

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2H-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-196a.M3

Plasmid

#220467

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7

Plasmid

#220468

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a

Plasmid

#220470

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7.M4

Plasmid

#220469

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-7

Plasmid

#220464

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-7.M4

Plasmid

#220465

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-196a

Plasmid

#220466

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al bioRxiv 2024.02.22.581357

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Use

Tags

Expression

Mutation

Promoter

Ubiquitin

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_18bp

Plasmid

#174852

Purpose

Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_18bp insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_18bp

Plasmid

#174853

Purpose

Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_18bp

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_44bp

Plasmid

#174854

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_44bp insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HEK3_pegR_44bp

Plasmid

#174855

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_44bp insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 641 - 660 of 12812 results

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