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pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Article

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-GCK

Plasmid

#23750

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GCK (GCK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-CSF1R

Plasmid

#23928

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSF1R (CSF1R Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FGFR2

Plasmid

#23904

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR2 (FGFR2 Human)

Use

Gateway donor vector

Mutation

A52D, T525A (compared to GenBank reference sequen…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCF806-shABC2

Plasmid

#186714

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF806-shABC1

Plasmid

#186713

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway donor vector

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FLT1

Plasmid

#23912

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway donor vector

Mutation

S755P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PAK1

Plasmid

#23543

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK1 (PAK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Human SHIP2

Plasmid

#124645

Purpose

Expresses HIS tagged human SHIP2 in COS-7/HEK cells

Depositor

Christophe Erneux

Article

Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

Insert

INPPL1 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Mammalian

Mutation

coding sequence starts at amino acid 18 as calcul…

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FGR

Plasmid

#23877

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGR (FGR Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DDR1

Plasmid

#23910

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR1 (DDR1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway donor vector

Mutation

Insert encodes truncated protein relative to NP_0…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK7

Plasmid

#23786

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK7 (CDK7 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway donor vector

Mutation

M74T V144E

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CARD11

Plasmid

#23482

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CARD11 (CARD11 Human)

Use

Gateway donor vector

Mutation

A291C,C292T,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway donor vector

Mutation

missing first codon

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only