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pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro

Plasmid

#141381

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro

Plasmid

#141389

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7b (ORF7b SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

Plasmid

#141377

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDC42_p.Q61L

Plasmid

#82174

Purpose

Gateway Donor vector containing CDC42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDC42 (CDC42 Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_S5E2_Chrimson_tdTomato

Plasmid

#219432

Purpose

AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

Depositor

Gordon Fishell

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chrimson-tdTomato

Use

AAV

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKACB

Plasmid

#20596

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKACB (PRKACB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCMV CHMP2B-L4D,F5D-M85E-3XHA

Plasmid

#242416

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M85E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D M85E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E M85E M92E-3XHA

Plasmid

#242418

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with V82E, M85E, and M92E mutations and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E M85E M92E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E-3XHA

Plasmid

#242393

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a V82E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-M92E-3XHA

Plasmid

#242417

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M92E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D M92E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d10-52-3xHA (Δα1)

Plasmid

#232004

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the first alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ10-52

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-96-3xHA (Δα2)

Plasmid

#232005

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d97-106-3xHA (Δα2/3 loop)

Plasmid

#232006

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the loop connecting the second and third alpha helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ97-106

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

Plasmid

#232007

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ106-113

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

Plasmid

#232008

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ118-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-138-3xHA (Δα2-4)

Plasmid

#232009

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second through fourth helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d159-174-3xHA (Δα5)

Plasmid

#232010

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fifth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ159-174

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d201-213-HA (ΔVPS4 binding)

Plasmid

#232011

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the VPS4-binding region deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

HA

Expression

Mammalian

Mutation

L4D F5D Δ201-213

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge