We narrowed to 118,634 results for: mpi

1. pPB-CAG-dCas9-VPR-wpA-pPuroTK

   Plasmid

   #214127

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-mito-dsRED2

   Plasmid

   #173071

   Purpose

   mitochondria targeted dsRED2 expressed off of CAG promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. THINGL pcDNA3.1

   Plasmid

   #230046

   Purpose

   Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   THINGL
   

   Expression

   Mammalian

   Available Since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK647

   Plasmid

   #71701

   Purpose

   Produces E. coli thioredoxin reductase (EcTrxB)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

   Plasmid

   #183249

   Purpose

   Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

   Depositor

   Alexander McLellan

   Insert

   Anti-Her2 scFvFc (FRP5)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AbVec_IGHC

   Plasmid

   #183701

   Purpose

   Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

   Depositor

   David Nemazee

   Insert

   IGHG1 (IGHG1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIX403-iRFP-ccdB-Puro

   Plasmid

   #158566

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   iRFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-XL4 FLAG-SFRP2-Fc

   Plasmid

   #115772

   Purpose

   Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.

   Depositor

   Davide Comoletti

   Insert

   SFRP2 (SFRP2 Human)
   

   Tags

   FLAG and hIgG/Fc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b-Casp1-His

    Plasmid

    #11809

    Depositor

    Guy Salvesen

    Insert

    Caspase-1 (CASP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T1-Opt WWP1

    Plasmid

    #179327

    Purpose

    E. coli optimized expression of GST-fusion of human WWP1

    Depositor

    Arthur Haas

    Insert

    WWP1 (WWP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pExp-Trx

    Plasmid

    #112577

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-TrxA and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    CB

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG CMV Empty Puro

    Plasmid

    #237881

    Purpose

    Empty mammalian expression plasmid for cloning untagged proteins

    Depositor

    Meghan Rego, Addgene Research Program

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid

    #230822

    Purpose

    AAV cloning vector for Flpo-dependent expression under the EF1a promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV; Cloning vector

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid

    #174893

    Purpose

    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Insert

    mCherry and EGFP
    

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-EGFP-p50

    Plasmid

    #107977

    Purpose

    Expresssion of NF-kB p50

    Depositor

    Georg Weber

    Insert

    NF-kB p50 (Nfkb1 Mouse)
    

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Amino acids VQACI added to C-terminus

    Promoter

    CMV

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2-HA-HshnRNPR_AA

    Plasmid

    #148235

    Purpose

    Mammalian Expression of HshnRNPR

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPR (HNRNPR Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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