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pDB5225

Plasmid

#215492

Purpose

A pUra4 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

Pil1-mCherry

Expression

Yeast

Promoter

P41nmt1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDB5227

Plasmid

#215494

Purpose

A pLys3 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

Pil1-mCherry

Expression

Yeast

Promoter

P41nmt1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDB5228

Plasmid

#215495

Purpose

A pHis5 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

Pil1-mCherry

Expression

Yeast

Promoter

P41nmt1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDB5253

Plasmid

#215501

Purpose

A pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1

Depositor

Li-Lin Du

Article

Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

Insert

mECitrine-CtNbr1

Expression

Yeast

Promoter

P41nmt1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 (1-122)

Plasmid

#210428

Purpose

Bacterial expression of MBP-PB1(1-122) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-122) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-RBP-WDR5

Plasmid

#209702

Purpose

Mammalian expression of WDR5 with N terminal RBP fusion

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

RBP-WDR5

Tags

RBP

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pT7RNAP-T15

Plasmid

#199991

Purpose

T7 RNAP circuit variant T15 on miniRK2 plasmid

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mCardinal

Plasmid

#197258

Purpose

Expresses the protein mCardinal in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-Clover

Plasmid

#209721

Purpose

Mammalian expression of clover localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-Clover

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5

Plasmid

#209706

Purpose

Mammalian expression of y-ATOM biosensor for WDR5

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_SH2

Plasmid

#209708

Purpose

Mammalian expression of y-ATOM biosensor for SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_SH2

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-SH2

Plasmid

#209717

Purpose

Mammalian expression of SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-SH2

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-NbGFP_miRFP670nano3

Plasmid

#209719

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-NbGFP_miRFP670nano3

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-iRFP682-N1

Plasmid

#197184

Purpose

Expresses the fusion protein of CytERM-iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-iRFP682

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-emiRFP703-N1

Plasmid

#197185

Purpose

Expresses the fusion protein of CytERM-emiRFP703 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-emiRFP703

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mRhubarb713-HO1

Plasmid

#197254

Purpose

Expresses the protein of mRhubarb713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb713

Expression

Bacterial

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCfuNca-Mito

Plasmid

#190819

Purpose

Mitochondria targeted GFP for diatoms

Depositor

Peter Kroth

Article

Sprecher et al Algal Research. 2023 May; Vol 72: 103136

Inserts

Diatom mitochondrial targeted GFP
Bleomycin resistance gene

Expression

Bacterial

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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vYM143_phiC31-nTRE-pEF1s(low)(CG793)-H2b-Citrine

Plasmid

#197686

Purpose

"pEF1s(low)" reporter plasmid, with CpG793 mutated to CG

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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