We narrowed to 16,276 results for: nes
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Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-tagBFP-KRAB
UseFish expressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT6471
Plasmid#219428PurposeModule of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)DepositorInsertAGGT_NLS-SpCas9i-NLS(no stop)_TTCG
UseMoclo compatible level 0 moduleAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mesp-1916/-1>nls::Cas9::nls
Plasmid#59988PurposeMesp driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Mesp -1916/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB5 (human) pBluescript
Plasmid#8531DepositorInsertHOXB5 (HOXB5 Human)
ExpressionBacterialMutationmakes protein in vitro transcription/translationAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSun1-2xsfGFP-6xMYC-pA
UseGateway CloningAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-N2cG
Plasmid#192838Purposeplasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorInsertmTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
UseAAVExpressionMammalianPromoterTREtightAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Duet1_6xHis-TEV-GABARAP-Gly
Plasmid#190930PurposePlasmid for the expression and purification of GABARAP. Internal Reference: SMC894DepositorAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRDX6-Halo
Plasmid#175338PurposeProduction of HaloTag fusion proteinDepositorPublicationAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin1
Plasmid#124865PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralPromoterH1, U6, 7SK, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…Available sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINDgw RFA-V5-EcoDam
Plasmid#59204PurposeMammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseDamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorPublicationAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flag-Avi-ires-CHD5
Plasmid#68878PurposeTransient mammalian expression of CHD5 for BirA taggingDepositorAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoECDS
Plasmid#41848DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertApoE CDS (APOE Human)
UseLuciferaseTagsrenilla luciferaseAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 HA (#1709)
Plasmid#14594DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLexA
TagsHA, Ubiquitin, and VP16ExpressionMammalianAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by