We narrowed to 14,582 results for: eng
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Plasmid#87333PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII
Plasmid#80862PurposePhagemid (encodes evolved cI5G6T,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6T,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGB 35s:dCas:VP64:tNos (GB1189)
Plasmid#75403PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional ActivatorDepositorInsertdCas:VP64
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX381 Filifactor alocis Cas9
Plasmid#68318PurposeFilifactor alocis Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFaCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX390 Flavobacterium columnare Cas9
Plasmid#68327PurposeFlavobacterium columnare Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFcCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorPublicationInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorPublicationInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p14-9VNA5
Plasmid#50931PurposeExpresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.DepositorTagsVN tag and flag tagExpressionMammalianMutationAmino acids 96-100 of SRP14 changed to alaninePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p14-9VN9-3A
Plasmid#50932PurposeExpresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.DepositorTagsVN tag and flag tagExpressionMammalianMutationAmino acids 60, 61 and 64 of SRP9 changed to alan…PromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermCan
Plasmid#51438PurposeExpresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Can C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermCan
Plasmid#51439PurposeExpresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Can C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN1
Plasmid#51445PurposeExpresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N1 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGC001
Plasmid#48824PurposeProvides WUS as GreenGate coding sequence module.DepositorInsertWUS (WUS Mustard Weed)
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition site removed by silent …Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
FVIII H2
Plasmid#46773PurposepcDNA4.full length FVIII D1241EDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfull length FVIII D1241E (F8 Human)
ExpressionMammalianMutationD1241EPromoterCMVAvailable sinceNov. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
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zgc:111821_R (OZ598)
Plasmid#35238DepositorInsertZinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)
UseZebrafish targetingAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only