279,377 results

1. pET16B.Pfu

   Plasmid

   #12509

   Purpose

   Bacterial expression of Pfu DNA polymerase

   Depositor

   Sung-Hou Kim

   Article

   Goldman et al Acta Crystallogr D Biol Crystallogr. 199

   Insert

   Pfu DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y218H, Q462R, F533L, K559I and V683A (mutations w…

   Promoter

   Available since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET16B.Pfu

   Plasmid

   #12509

   Purpose

   Bacterial expression of Pfu DNA polymerase

   Depositor

   Sung-Hou Kim

   Article

   Goldman et al Acta Crystallogr D Biol Crystallogr. 199

   Insert

   Pfu DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y218H, Q462R, F533L, K559I and V683A (mutations w…

   Promoter

   Available since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Use

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73022

   Purpose

   Luminescent and fluorescent dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73022

   Purpose

   Luminescent and fluorescent dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-Sox10MCS5-GFP

   Plasmid

   #115783

   Purpose

   This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Insert

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   

   Use

   Lentiviral

   Tags

   cfos basal promoter conjugated MCS5 promoter fuse…

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV-Sox10MCS5-GFP

   Plasmid

   #115783

   Purpose

   This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Insert

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   

   Use

   Lentiviral

   Tags

   cfos basal promoter conjugated MCS5 promoter fuse…

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-Flag-hS1pr1-GFP-Neo

   Plasmid

   #203708

   Purpose

   Overexpression of FLAG-S1PR1-GFP fusion.

   Depositor

   Timothy Hla

   Insert

   Flag-hS1PR1-GFP (S1PR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-TAG-NICD

   Plasmid

   #130934

   Purpose

   PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   NICD (NOTCH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-TAG-NICD

    Plasmid

    #130934

    Purpose

    PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NICD (NOTCH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. All-in-one lenti-evoCDA-NG

    Plasmid

    #200449

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    myc, His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    myc, His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVpuro-mTagBFP2

    Plasmid

    #96935

    Purpose

    Retroviral plasmid containing mTagBFP2 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Monomeric Tag Blue Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVpuro-mTagBFP2

    Plasmid

    #96935

    Purpose

    Retroviral plasmid containing mTagBFP2 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Monomeric Tag Blue Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-U6

    Plasmid

    #120358

    Purpose

    piggyBac transposon vector for sgRNA expression.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PB-U6

    Plasmid

    #120358

    Purpose

    piggyBac transposon vector for sgRNA expression.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-Rho*-BERKY3

    Plasmid

    #158220

    Purpose

    BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-Rho*-BERKY3

    Plasmid

    #158220

    Purpose

    BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMyBADC-kan

    Plasmid

    #84689

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMyBADC-kan

    Plasmid

    #84689

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge