We narrowed to 7,293 results for: Ank

1. pLV.5’VIM-eGFP-KI

   Plasmid

   #170547

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Inserts

   Left Homology arm for VIM knockin
   Right Homology arm for VIM knockin
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Tubg1-mEGFP

   Plasmid

   #196870

   Purpose

   Expression of the gamma-tubulin fused to enhanced (E)-GFP

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Tubg1-mEGFP (Tubg1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLenti6-DCX-EGFP

   Plasmid

   #80599

   Purpose

   pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

   Depositor

   Torsten Wittmann

   Article

   Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

   Insert

   Doublecortin (DCX Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-AAVS1-PGK-Puro-moxGFP

   Plasmid

   #227275

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKC12 - DelQ

   Plasmid

   #110493

   Purpose

   Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

   Depositor

   Shankar Balasubramanian

   Article

   Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

   Insert

   NRAS 5'- DelQ (bp 30-254) (NRAS Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Mutation

   Deletion of the first 29 base pairs of the NRAS 5…

   Promoter

   T7

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC28-Bsa4_ZV-NS5-RdRp

   Plasmid

   #179847

   Purpose

   The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

   Depositor

   Julien Lescar

   Article

   Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

   Insert

   Zika Virus Non-structural protein 5 RdRp domain
   

   Tags

   6-histine

   Expression

   Bacterial

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-lox-roxSTOProx-tRFP-pA-lox

   Plasmid

   #196888

   Purpose

   Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   turbo (t)RFP
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

    Plasmid

    #196889

    Purpose

    Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    lyn-mScarlet
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ins:GCaMP6s; cryaa:RFP

    Plasmid

    #110285

    Purpose

    A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Insert

    GCaMP6s
    

    Use

    Zebrafish

    Promoter

    insulin

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCLc-MND-B2705-SABR-Backbone

    Plasmid

    #119051

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid

    #191334

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVd1-EGFP-hRhoA-wt

    Plasmid

    #133926

    Purpose

    Low level expression of GFP fused human RhoA wild type

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    GFP-hRhoA WT (RHOA Human)
    

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScarlessHD-C-3xVHH05-DsRed

    Plasmid

    #171580

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBC-LG3-tdT

    Plasmid

    #62810

    Purpose

    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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