We narrowed to 7,696 results for: CCH

1. pPK-351

   Plasmid

   #157921

   Purpose

   pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   PIF3-MTAD
   IRES
   PhyB(1-621)-SV40NLS-Gal4DBD
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   HA tag and SV40NLS

   Expression

   Mammalian

   Mutation

   pif3 1-523

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc-HT-Dmp53KRKR

   Plasmid

   #85699

   Purpose

   expression of Drosophila p53 KRKR mutant in Drosophila cells

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Tags

   His

   Expression

   Insect

   Mutation

   K26R K302R

   Promoter

   Ac5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH399-SUP45-S30P

   Plasmid

   #29372

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   S30P mutant gene (corresponding to the sl23ts all…

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pTH375-SUP45-P174Q

   Plasmid

   #29383

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   P174Q mutant gene including genomic sequence 1432…

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Expression

   Yeast

   Mutation

   See depositor comments below

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS316-VTC4

   Plasmid

   #108691

   Purpose

   VTC4 sequence expressed in yeast plasmid

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Vacuolar Transporter Chaperone 4
   

   Expression

   Yeast

   Promoter

   VTC4 (native promoter)

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCOG75-Sem1 Sc

   Plasmid

   #83233

   Purpose

   pCDF-His6-MBP-Sem1 Sc

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   SEM 1 (SEM1 Budding Yeast)
   

   Tags

   His-mbp

   Expression

   Bacterial

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3037(XI-5 MarkerFree)

    Plasmid

    #73277

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

    Plasmid

    #86818

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAG415-ADH1p-HA-rap1-K9R

    Plasmid

    #84531

    Purpose

    This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    ADH1

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pAG415-GPDp-EGFP-rap1-K3NR

    Plasmid

    #84533

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,117,118)R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pAG415-GPDp-EGFP-rap1-K3CR

    Plasmid

    #84534

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(462,463,651)R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAG415-GPDp-EGFP-rap1-K9R

    Plasmid

    #84535

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K462-463-651R

    Promoter

    none

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K43-117-118R

    Promoter

    none

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGS1212

    Plasmid

    #63785

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Strep-tag II

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS424-TUB2 - human TUBB6 Cterminal tail

    Plasmid

    #60403

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only