We narrowed to 24,081 results for: crispr

Showing: 6661 - 6680 of 24081 results

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qTAG-C-mScarlet-Blast

Plasmid

#207711

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mScarlet

Plasmid

#207689

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Puro

Plasmid

#207722

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-gRNA-puro

Plasmid

#180426

Purpose

Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-mStayGold

Plasmid

#227252

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-V5-Puro

Plasmid

#207730

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Blast

Plasmid

#207717

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa-VPR mini.

Plasmid

#99685

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-3xHA

Plasmid

#207696

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p2CT-His-MBP-Hhe_Cas13a_WT

Plasmid

#91871

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

HheCas13a (HheC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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