We narrowed to 13,416 results for: sequence
-
Plasmid#174907PurposeSecond generation sgRNA against Sp1DepositorInsertSpecificity Protein 1 (SP1 Human)
ExpressionMammalianMutationMissense mutation to mutate PAM sequencePromoterEFS-NSAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC_RR_VP1_r1c3
Plasmid#65724PurposeRescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSRDepositorInsertmCherry
UseAAVAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-4mtGCaMP6f-DIO
Plasmid#179529PurposeCre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoterDepositorInsert4mtGCaMP6f
UseAAVTags4x Cox8 targeting sequencePromoterEf1aAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-63: ACTN2-mEGFP
Plasmid#124607PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM-intron 3x UAS attB
Plasmid#206360Purposeincludes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequencesDepositorTypeEmpty backboneExpressionInsectAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-43: GJA1-mEGFP
Plasmid#109120PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1DepositorInsertGJA1 Homology Arms with linker-mEGFP (GJA1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-41: ATP2A2-mEGFP
Plasmid#114405PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2DepositorInsertATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRExpressionWormAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
AICSDP-47: CTNNB1-mEGFP
Plasmid#109119PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1DepositorInsertCTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcNDA-NES-PhoCl2c-mCherry
Plasmid#164037PurposeOptogenetic control of protein localization by PhoCl2cDepositorInsertNES-PhoCl2c-mCherry
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HRE-doUnaG
Plasmid#201713PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequenceDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME phluorin2-gpi
Plasmid#73793Purposeratiometric pHluorin2 with a gpi linkage sequence in a middle entry cloneDepositorInsertratiometric pHluorin2-gpi
UseMiddle entry cloneAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
1.3xWT HBV-Luciferase
Plasmid#61549PurposeThe 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of CoreDepositorInsert1.3 times of the HBV genome with luciferase within the Core ORF
UseLuciferaseExpressionMammalianMutationSubstitution of HBV sequences with luciferase ORF…Available SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
ExpressionMammalianAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
Halo-DNAPKcs HRD
Plasmid#207088PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1(P004)
Plasmid#184056PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.DepositorInsertA1-20B1 preamplifier
UseSynthetic BiologyAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]
Plasmid#133327PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS:iGluSnfr.zf1
Plasmid#108356PurposeUAS driven variant of iGluSnFR for zebrafish expressionDepositorInsertUAS:[GlyRa1 secretion sequence]-[iGluSnFR]
UseZebrafish expressionPromoterlacAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only