We narrowed to 41,408 results for: CHI

1. pInducer20 CARD8-HA PAM

   Plasmid

   #169982

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-RdLight1

   Plasmid

   #125707

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Has Service

   AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dLight1.2

   Plasmid

   #111054

   Purpose

   Expresses dLight1.2 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.2
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas-CmR(+)

   Plasmid

   #113633

   Purpose

   Chloramphenicol resistant derivative of pCas plasmid

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   Chloramphenicol resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Pcat

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-hUba2-His

   Plasmid

   #53117

   Purpose

   expression of full length human Uba2 with Cterminal His-tag in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Methods Mol Biol. 2009;497:187-99. doi:

   Insert

   UBA2 (UBA2 Human)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 NLRP1

   Plasmid

   #166823

   Purpose

   Gateway pDONR vector containing NLRP1 (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Stat1 beta Flag pRc/CMV

   Plasmid

   #8704

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   STAT1 beta (STAT1 Human)
   

   Use

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

9. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule-para-aminoPhe

    Plasmid

    #85502

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP CMV VHHG4

    Plasmid

    #182236

    Purpose

    Expresses GFP nanobody for stable expression in mammalian cells

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-Myc-IRES-GFP

    Plasmid

    #35396

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

    Inserts

    Myc (Myc Mouse)
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. dTAG NLRP1

    Plasmid

    #166825

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR P4-P1R-ECFP

    Plasmid

    #48352

    Purpose

    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330a dCas9-VP64

    Plasmid

    #92363

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Stat1 alpha pRC/CMV

    Plasmid

    #8690

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only