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mCherry-miniSOG-Talin-N-10

Plasmid

#55096

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Talin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean-Zyxin-6

Plasmid

#55398

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Zyxin (ZYX Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Talin-ABM-10

Plasmid

#55510

Purpose

Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Talin ABM

Tags

mTFP1

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Profilin-10

Plasmid

#55093

Purpose

Localization: Cytoskeleton, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Profilin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F Y96F

Plasmid

#47092

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5 Flag-NFRKB delta101

Plasmid

#19790

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

deleted N-terminal 1-101 amino acids

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pIS57 PGM2 3'UTR mut

Plasmid

#14492

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

PGM2 3'UTR mutant (PGM2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS56 NETO2 3'UTR mut

Plasmid

#14496

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

NETO2 3'UTR mutant (NETO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS55 POGK 3'UTR mut

Plasmid

#14498

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

POGK 3'UTR mutant (POGK Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

First, fourth, and fifth occurrences of CATTCC’s …

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTmpl-miniDonor-GFP

Plasmid

#238107

Purpose

Mini donor expressing GFP, used for PCR to generate IVT template.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS_3E5E_eGFP

Plasmid

#103054

Purpose

Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

Ebola virus (ebolavirus Zaire, Mayinga) minigenome

Tags

3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Plasmid

#226514

Purpose

Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag_fast

Plasmid

#167275

Purpose

Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_355

Plasmid

#187159

Purpose

Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

Depositor

John Doench, David Root

Article

Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSM41

Plasmid

#235728

Purpose

Protein expression of FL His6- human VPS34 in bacteria

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

VPS34 (PIK3C3 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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