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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,984 results for: nes

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Showing: 6701 - 6720 of 18984 results

  1. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Publication
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    182958-plasmid-map-sequence-id-365360

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  2. SMYD3

    Plasmid
    #25318
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3_26 (SMYD3 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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  4. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    73220-plasmid-map-sequence-id-134711

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  10. RP2-EGFP

    Plasmid
    #86073
    Purpose
    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RP2 (RP2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86073-plasmid-map-sequence-id-168962

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  15. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  17. NRDP1

    Plasmid
    #25620
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRDP1 (RNF41 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains USP8 interaction Domain
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

    Plasmid
    #196191
    Purpose
    Tagging human SOX2 gene with P2A-EGFP at the C-terminus
    Depositor
    Jürgen Knoblich
    Publication
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
    Use
    Homology repair donor
    Mutation
    G2913A mutation to replace the guide cutting site…
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196191-plasmid-map-sequence-id-384896

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Showing: 6701 - 6720 of 18984 results