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pICSL50008

Plasmid

#50314

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAL197

Plasmid

#22280

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-PakGBD-mCherry

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pICSL50007

Plasmid

#50308

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal FLAG tag

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80007

Plasmid

#50323

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP11

Plasmid

#80666

Purpose

Encodes the synthetic coiled coil peptide SYNZIP11

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP11

Use

Gateway Cloning

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAL198

Plasmid

#22281

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-mCherry-WaspGBD

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Article

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Tags

His8 tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pICH42211

Plasmid

#50255

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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