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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,379 results for: Cre

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Showing: 6721 - 6740 of 14379 results

  1. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid
    #169182
    Purpose
    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169182-plasmid-map-sequence-id-333999

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  2. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  4. Dom neg CstF64

    Plasmid
    #127250
    Purpose
    Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation
    Depositor
    Christine Milcarek
    Publication
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CstF64 delta 282 (CSTF2 Human)
    Use
    Other
    Expression
    Mammalian
    Mutation
    insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127250-plasmid-map-sequence-id-250029

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  5. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid
    #113496
    Purpose
    Expression of human Ataxin-3 with C-terminal strep-HA tag
    Depositor
    Hemmo Meyer
    Publication
    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ataxin-3 (ATXN3 Human)
    Tags
    2xstrep-HA
    Expression
    Mammalian
    Mutation
    wildtype without stop codon
    Promoter
    CMV
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113496-plasmid-map-sequence-id-218585

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  8. nes1852tk/lacZ

    Plasmid
    #47613
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Publication
    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nestin 2nd intron (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Mutation
    2nd intron only
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimised EBA140
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    47742-plasmid-map-sequence-id-68750

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  15. PTEX150-bio

    Plasmid
    #47788
    Purpose
    Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimised PTEX150
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47788-plasmid-map-sequence-id-68825

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  20. PB-CT3G-CERP2

    Plasmid
    #175499
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Publication
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175499-plasmid-map-sequence-id-345877

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Showing: 6721 - 6740 of 14379 results