We narrowed to 18,492 results for: REV

1. Kif3A-EGFP-SNAP

   Plasmid

   #229852

   Purpose

   Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif3A (Kif3a Mouse)
   

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

   Plasmid

   #231592

   Purpose

   Express circular RNA translation reporter with 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. N-Terminal Halo XRCC4

   Plasmid

   #207541

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-terminal Halo-Ku70

   Plasmid

   #207547

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with flanked by human Ku70 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xMS-TR-100-PURO HRD

   Plasmid

   #207582

   Purpose

   homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
   

   Tags

   3xMS2

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac128-TEF2p-LDH-CYC1t

   Plasmid

   #216120

   Purpose

   Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Lactate dehydrogenase (LDH Bovine)
   

   Expression

   Yeast

   Promoter

   TEF2 promoter

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac204-TDH3p-LDH-CYC1t

   Plasmid

   #216121

   Purpose

   Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Lactate dehydrogenase (LDH Bovine, Bos taurus)
   

   Expression

   Yeast

   Promoter

   TDH3 promoter

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tensin-2 Y483E

   Plasmid

   #201794

   Purpose

   Expresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backbone

   Depositor

   Vesa Hytönen

   Article

   Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

   Insert

   Tensin-2 (Tns2 Mouse)
   

   Tags

   BirA, HA, strepIII

   Expression

   Bacterial and Mammalian

   Mutation

   mutation Y483E

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tensin-2 Y483F

    Plasmid

    #201795

    Purpose

    Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483F

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tensin-2 Y483A

    Plasmid

    #201796

    Purpose

    Expresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483A

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM71

    Plasmid

    #227621

    Purpose

    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 24-160-GGSGSG-192-221

    Promoter

    T7

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP-AUA-IRES-mCherry

    Plasmid

    #222116

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AUA start codon

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-AGC-IRES-mCherry

    Plasmid

    #222119

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AGC start codon

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHM148

    Plasmid

    #225532

    Purpose

    Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    rtTA
    Pcdh19 (Pcdh19 Mouse)
    mCherry
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Mito(OM)-deltaCKAR

    Plasmid

    #222431

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Mitochondrial outer membrane delta-selective C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-NUDT5

    Plasmid

    #219955

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    NUDT5 (NUDT5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST17-LAP3

    Plasmid

    #217661

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    LAP3 (LAP3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-LAP3

    Plasmid

    #217660

    Purpose

    For gateway cloning of LAP3

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    LAP3 (LAP3 Human)
    

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-AGG-NLS-EGFP

    Plasmid

    #214602

    Purpose

    express EGFP ; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    EGFP
    

    Use

    Unspecified

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT7-AGG-SpCas9

    Plasmid

    #214603

    Purpose

    encode SpCas9; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    SpCas9
    

    Use

    CRISPR and Unspecified

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGFP-CUG-IRES-mCherry

    Plasmid

    #222110

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    CUG start codon

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGFP-ACG-IRES-mCherry

    Plasmid

    #222113

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    ACG start codon

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGFP-UUG-IRES-mCherry

    Plasmid

    #222112

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    UUG start codon

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGFP-AUU-IRES-mCherry

    Plasmid

    #222118

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AUU start codon

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGFP-AGG-IRES-mCherry

    Plasmid

    #222115

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AGG start codon

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGFP-AUC-IRES-mCherry

    Plasmid

    #222117

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AUC start codon

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSET-Cm-CspA1

    Plasmid

    #215398

    Purpose

    Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA1
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli BL21(DE3)

    Promoter

    T7 promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE

    Plasmid

    #216828

    Purpose

    Expresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

    Insert

    Tornado-Squash-FTL-IRE
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE

    Plasmid

    #216829

    Purpose

    Expresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

    Insert

    Tornado-Squash-TfR-IRE
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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