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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,526 results for: Tet-on

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Showing: 6761 - 6780 of 7526 results

  1. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    DsRed
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

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  3. IBMc326

    Plasmid
    #161949
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161949-plasmid-map-sequence-id-327545

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  4. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  5. pFAB1935

    Plasmid
    #47860
    Purpose
    BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    his_T_min_linker_crp_T_min double terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    concatenate of his_min and crp_min
    Promoter
    pLTetO15
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47860-plasmid-map-sequence-id-68392

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  11. JPF0419v

    Plasmid
    #124021
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124021-plasmid-map-sequence-id-262675

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  12. pMT3-RhoAC

    Plasmid
    #100032
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoAC
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    E497K/C566D
    Promoter
    Adenovirus major late promoter
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100032-plasmid-map-sequence-id-193105

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  14. pMT3-RhoGC E254D

    Plasmid
    #100033
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC E254D
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    E254D
    Promoter
    Adenovirus major late promoter
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  15. pFAB1902

    Plasmid
    #47858
    Purpose
    BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    M13_central_T_linker_rrnD_T1 double terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    concatenate of M13 central and rrnD
    Promoter
    pLTetO13
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47858-plasmid-map-sequence-id-68390

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  17. pCIFR-5

    Plasmid
    #233294
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Promoter
    p14G-BCD2
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233294-plasmid-map-sequence-id-468478

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  18. pFNC-5

    Plasmid
    #233297
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233297-plasmid-map-sequence-id-468477

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Showing: 6761 - 6780 of 7526 results