We narrowed to 11,723 results for: Vars

1. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223 SARS-CoV-2 ORF9B_nostop

   Plasmid

   #149327

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDONR223_REG1A_WT

   Plasmid

   #81785

   Purpose

   Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   REG1A (REG1A Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_SMAD3_WT

   Plasmid

   #82272

   Purpose

   Gateway Donor vector containing SMAD3, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 SARS-CoV-2 ORF9Bwu

   Plasmid

   #149331

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9Bwu
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR223_NFKBIA_WT

   Plasmid

   #81833

   Purpose

   Gateway Donor vector containing NFKBIA ,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NFKBIA (NFKBIA Human)
   

   Use

   Gateway entry vector

   Mutation

   S76P

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_U2AF1_WT

   Plasmid

   #81954

   Purpose

   Gateway Donor vector containing U2AF1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CARD14-E138A

   Plasmid

   #69921

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   E138A

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223 SARS-CoV2 TEV-NSP12

   Plasmid

   #154410

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_PIK3CG_WT

    Plasmid

    #81843

    Purpose

    Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CG (PIK3CG Human)
    

    Use

    Gateway entry vector

    Mutation

    E2G

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_PRDM1_WT

    Plasmid

    #81761

    Purpose

    Gateway Donor vector containing PRDM1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_OMA1_WT

    Plasmid

    #81889

    Purpose

    Gateway Donor vector containing OMA1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    OMA1 (OMA1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_TSC2_WT

    Plasmid

    #82281

    Purpose

    Gateway Donor vector containing TSC2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Gateway entry vector

    Mutation

    A210V; K704E; L1015M; I1649M

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid

    #169336

    Purpose

    Expression of the LT-Fucci(SA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_U2AF1_p.S34F

    Plasmid

    #81358

    Purpose

    Gateway Donor vector containing U2AF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    S34F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_POT1_p.G76V

    Plasmid

    #81497

    Purpose

    Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    POT1 (POT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    G76V

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223 SARS-CoV2 TEV-NSP5

    Plasmid

    #154404

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR223 SARS-CoV2 TEV-S

    Plasmid

    #154415

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of s…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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