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6975 pPROEX-HTb-SIS1

Plasmid

#1233

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

sis1 (SIS1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER.MRE11i

Plasmid

#15566

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-start

Plasmid

#127168

Purpose

Entry plasmid for two-step guide-barcode cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

N/A

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro

Plasmid

#60912

Purpose

Enocdes Camsap1L1 fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Camsap1L1

Expression

Mammalian

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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CF-iSH

Plasmid

#20159

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-p85 (Pik3r2 Mouse)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pBAD-D4

Plasmid

#37473

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D4 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pMAH-ER-bapaUGAc

Plasmid

#201674

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

ER-bapaUGAc

Tags

ER leading sequence and ER retention sequence

Expression

Mammalian

Promoter

CMV SP6

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRec

Plasmid

#104572

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway donor vector

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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