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Plasmid#78694PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RFXANK_2
Plasmid#86365PurposeEncodes gRNA for 3' target of human RFXANKDepositorInsertgRNA against RFXANK (RFXANK Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RFXANK_1
Plasmid#86364PurposeEncodes gRNA for 3' target of human RFXANKDepositorInsertgRNA against RFXANK (RFXANK Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NCoA2_1
Plasmid#86341PurposeEncodes gRNA for 3' target of human NCoA2DepositorInsertgRNA against NCoA2 (NCOA2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF644_2
Plasmid#86306PurposeEncodes gRNA for 3' target of human ZNF644DepositorInsertgRNA against ZNF644 (ZNF644 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF644_1
Plasmid#86305PurposeEncodes gRNA for 3' target of human ZNF644DepositorInsertgRNA against ZNF644 (ZNF644 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL671
Plasmid#62825PurposeLim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsglgC leaderExpressionBacterialMutationPromoterpConAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1017
Plasmid#62828PurposeLim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsm8::glgC leaderExpressionBacterialMutationPromoterpConNoHindAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags3XFLAG-Cas9ExpressionMammalianMutationPromoterCBh; U6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFCIV-CEACAM6
Plasmid#149303Purposelentiviral packaging vector for CEACAM6 expression.DepositorInsertCEACAM6 (CEACAM6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterubiquitin CAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTH-GFP
Plasmid#99128PurposeAn AAV genome that expresses GFP from the mouse TH promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromotermTHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFLARE2
Plasmid#158700PurposeNeuronal expression of scFLARE2 componentDepositorInsertscFLARE2
UseAAVTagsHA, Flag, VP16ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterSynAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-CD19 puro
Plasmid#196634PurposeExpress CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-sec61b
Plasmid#186959PurposeExpress sec61b connected with N-terminus HaloTagDepositorInsertSec61 (SEC61B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mTurquoise2
Plasmid#99122PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoterDepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHDM-VSV-G
Plasmid#164440PurposeEncodes VSV-G protein for pseudovirus productionDepositorInsertVSV-G
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW240
Plasmid#170791PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolutionDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_TBX5_P2A_Hygro_Barcode
Plasmid#120486PurposeBarcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-ER-3
Plasmid#186960PurposeER lumen localizaing HaloTagDepositorInsertCALR (CALR Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mTurquoise2
Plasmid#99125PurposeAn AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoterDepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mNeonGreen
Plasmid#99135PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoterDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG_hPPP1R15B_4-713_mCherry_UK1298
Plasmid#80707Purposemammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)DepositorInserthuman PPP1R15B (PPP1R15B Human)
UseTagsmCherryExpressionMammalianMutationCDS 4-713 no stop codonPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only