Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 2,347 results for: puc19

Showing: 1651 - 1700 of 2347 results

  3. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

    Enlarge

  9. pLib6.6

    Plasmid
    #176652
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176652-plasmid-map-sequence-id-348545

    Enlarge

  11. pLib6.4B

    Plasmid
    #176665
    Purpose
    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176665-plasmid-map-sequence-id-348636

    Enlarge

  13. pLib6.6B

    Plasmid
    #176666
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176666-plasmid-map-sequence-id-348639

    Enlarge

  16. pIRTetR-GAL80

    Plasmid
    #185853
    Purpose
    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185853-plasmid-map-sequence-id-370762

    Enlarge

  17. pILGFP3A2 (2)

    Plasmid
    #185852
    Purpose
    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185852-plasmid-map-sequence-id-370761

    Enlarge

  20. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

    Plasmid
    #110831
    Purpose
    Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    Azgp1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CMV
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110831-plasmid-map-sequence-id-214111

    Enlarge

  28. pML2(−)WF

    Plasmid
    #108337
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108337-plasmid-map-sequence-id-209244

    Enlarge

  32. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Use
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87247-plasmid-map-sequence-id-173228

    Enlarge

  34. pML2(+)BR

    Plasmid
    #108336
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108336-plasmid-map-sequence-id-214578

    Enlarge

  35. pML2(+)WF

    Plasmid
    #108333
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108333-plasmid-map-sequence-id-209522

    Enlarge

  36. pML2(+)BF

    Plasmid
    #108334
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108334-plasmid-map-sequence-id-209526

    Enlarge

  37. pML2(+)WR

    Plasmid
    #108335
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108335-plasmid-map-sequence-id-209243

    Enlarge

  38. pML2(−)BF

    Plasmid
    #108338
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108338-plasmid-map-sequence-id-209524

    Enlarge

  39. pML2(−)WR

    Plasmid
    #108339
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108339-plasmid-map-sequence-id-209534

    Enlarge

  40. pML2(−)BR

    Plasmid
    #108340
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108340-plasmid-map-sequence-id-209245

    Enlarge

  46. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

    Enlarge

Showing: 1651 - 1700 of 2347 results