We narrowed to 3,255 results for: GAI

1. pSFFV-dNme2Cas9

   Plasmid

   #193190

   Purpose

   Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   dNme2Cas9
   

   Use

   Tags

   NLS and NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   H588A, D16A

   Promoter

   SFFV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/TO/FLAG/HA-DEST PABPC4

   Plasmid

   #19882

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-6011nes

   Plasmid

   #108652

   Purpose

   An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-ERK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVk7-33G1-Flag

   Plasmid

   #58950

   Purpose

   backbone for mouse recombinant antibody with added C-terminal FLAG tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT/FLAG/HA-DEST QKI

   Plasmid

   #19891

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVk7-33G1-BioLHis

   Plasmid

   #58949

   Purpose

   backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   6His tag and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCNL-N1_vinculin

   Plasmid

   #65691

   Purpose

   Expresses Vinculin-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   vinculin (Vcl Mouse)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCSIIbsr-6013x

   Plasmid

   #108654

   Purpose

   An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   Raichu-EV
   

   Use

   Lentiviral

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-HA-tdMCP-tdTomato

   Plasmid

   #183938

   Purpose

   tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdMCP-tdTomato (cp Synthetic)
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR4 TNRC6B

    Plasmid

    #20022

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6B (TNRC6B Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGLTR-X-PURO

    Plasmid

    #58246

    Purpose

    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Insert

    Puro(R)
    

    Use

    Lentiviral and RNAi; Gateway destionation vector

    Tags

    TetR-NLS-T2A

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-6020nes

    Plasmid

    #108656

    Purpose

    A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-JNK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. rTetR-GFP-VP16

    Plasmid

    #183917

    Purpose

    VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    rTetR-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTorPE-G-GECO1.2

    Plasmid

    #32468

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSb_init containing the TM287/288 nanobody Nb_TM#2

    Plasmid

    #132698

    Purpose

    expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    nanobody Nb_TM#2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-mGreenLantern/EGFP

    Plasmid

    #179913

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α-DIO-SN

    Plasmid

    #181741

    Purpose

    AAV vector for expressing SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE Zeo IkB SR

    Plasmid

    #83023

    Purpose

    Retroviral expression of dominant negative IkB alpha

    Depositor

    Yi Zhang

    Article

    Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

    Insert

    NFKB Inhibitor Alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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