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pORANGE GFP-Arpc5 KI

Plasmid

#131503

Purpose

Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Arpc5 KI

Plasmid

#131503

Purpose

Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SFTPB Promoter reporter

Plasmid

#88868

Purpose

Surfactant Protein B Gene Promoter Reporter

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Surfactant protein B gene promoter (SFTPB Human)

Use

Luciferase

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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SFTPB Promoter reporter

Plasmid

#88868

Purpose

Surfactant Protein B Gene Promoter Reporter

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Surfactant protein B gene promoter (SFTPB Human)

Use

Luciferase

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-RAB11a KI

Plasmid

#131499

Purpose

Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rab11a, Rab11a Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-RAB11a KI

Plasmid

#131499

Purpose

Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rab11a, Rab11a Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Pclo KI

Plasmid

#139657

Purpose

Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Pclo KI

Plasmid

#139657

Purpose

Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-surrogate-reporter

Plasmid

#65624

Purpose

Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attP

Use

Bxb1

Expression

Mammalian

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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Bxb1-surrogate-reporter

Plasmid

#65624

Purpose

Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attP

Use

Bxb1

Expression

Mammalian

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hVEGFA

Plasmid

#112553

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

Insert

VEGFA (VEGFA Human)

Use

Luciferase

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hVEGFA

Plasmid

#112553

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

Insert

VEGFA (VEGFA Human)

Use

Luciferase

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE Shank1-GFP KI

Plasmid

#131500

Purpose

Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Shank1-GFP KI

Plasmid

#131500

Purpose

Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge