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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,112 results for: cas12a

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Showing: 661 - 680 of 1112 results

  1. pMaCTag-Z09

    Plasmid
    #120052
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus
    Use
    Pcr template
    Tags
    mVenus
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120052-plasmid-map-sequence-id-232661

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  4. pMaCTag-P17

    Plasmid
    #120028
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEos4b
    Use
    Pcr template
    Tags
    mEos4b
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120028-plasmid-map-sequence-id-232635

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  5. pMaCTag-P08

    Plasmid
    #120019
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mClover3
    Use
    Pcr template
    Tags
    mClover3
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120019-plasmid-map-sequence-id-232617

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  7. pMaCTag-15

    Plasmid
    #119994
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miRFP670
    Use
    Pcr template
    Tags
    miRFP670
    Promoter
    None
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119994-plasmid-map-sequence-id-232514

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  11. U6-5' MS2-CircRNA

    Plasmid
    #213750
    Purpose
    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213750-plasmid-map-sequence-id-422195

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  16. gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217341
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217341-plasmid-map-sequence-id-426767

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  18. pUPD2 HDV (GB1444)

    Plasmid
    #160576
    Purpose
    Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.
    Depositor
    Diego Orzaez
    Publication
    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HDV
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160576-plasmid-map-sequence-id-322085

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  20. pMaCTag-Z03

    Plasmid
    #120046
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    moxCerulean3
    Use
    Pcr template
    Tags
    moxCerulean3
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120046-plasmid-map-sequence-id-232652

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Showing: 661 - 680 of 1112 results