We narrowed to 5,007 results for: puc
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Plasmid#170919PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
tagRFP-IMBB4-pUC
Plasmid#170769Purposetest plasmid for 5RM assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-POLBHR-eGFP
Plasmid#176064PurposeHomology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1DepositorInsertEGFP-PolB (POLB Human)
UseTagsEGFPExpressionMammalianMutationpUC with endogenous SapI mutated out, PAM mutatio…PromoterN/AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-MT-C34
Plasmid#177153PurposeLentiviral vector for expression of anti-HIV-1 MT-C34 peptide on cell surfaceDepositorInsertmClover-MT-C34
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-mEGFP
Plasmid#160469PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to mEGFPDepositorInsertppSEC13-mEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-msGFP2
Plasmid#160470PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2DepositorInsertppSEC13-msGFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK M15 (CUA)
Plasmid#154784Purposeplasmid with single copy of Mma PylT M15 mutant (CUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationG10A, G14U, G16U, C20U, C52APromoterh7SKAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK M15 (UUA)
Plasmid#154785Purposeplasmid with single copy of Mma PylT M15 mutant (UUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationUUA anticodon and G10A, G14U, G16U, C20U, C52APromoterh7SKAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R1R4
Plasmid#141054PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R5R4
Plasmid#141055PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(G:H)
Plasmid#138259PurposeExpression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter systemDepositorInsertmCherry targeting sgRNAs G and H
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(NT:NT)
Plasmid#138260PurposeExpression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WTdel6)
Plasmid#111324Purposefor T7 transcription of wt actin pre-mRNA (del6)DepositorInsertACT1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(AcAc)
Plasmid#111327PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAc mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrG)
Plasmid#111326PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to G mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrC)
Plasmid#111325PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to C mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap
Plasmid#49158PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap onlyDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and downstream sequence have been del…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only