We narrowed to 150,751 results for: ins

1. pET32a-hAREG

   Plasmid

   #199229

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Amphiregulin preproprotein (AREG Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-2XHA-Src-KD

   Plasmid

   #140312

   Purpose

   Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Contains only the KD region

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. P41-bio

   Plasmid

   #47739

   Purpose

   Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised P41
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRuby2-Keratin-17

   Plasmid

   #55900

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Keratin (KRT18 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

   Plasmid

   #192471

   Purpose

   Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Tags

   AcGFP1

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZIKV_NASBA_32B

   Plasmid

   #75011

   Purpose

   Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_NASBA_32B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Zap70(C)-SH2

   Plasmid

   #46533

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Zap70(C) (ZAP70 Human)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 154-267

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCST-AausFP4

   Plasmid

   #129502

   Purpose

   Expresses AausFP4 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP4
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. STK16_HUMAN_D0

    Plasmid

    #79701

    Purpose

    This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK16 (STK16 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. ZIKV_NASBA_27B

    Plasmid

    #75010

    Purpose

    Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_27B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKK-TEV-FLAG

    Plasmid

    #105783

    Purpose

    Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX CblC-SH2

    Plasmid

    #46414

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cblc CBLC (CBLC Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-326

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-2XHA-Src-S4-UD

    Plasmid

    #140304

    Purpose

    Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and UD regions

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST40-2XHA-Src-S4-KD

    Plasmid

    #140296

    Purpose

    Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST40-2XHA-Src-S4-UD-KD

    Plasmid

    #140300

    Purpose

    Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the SH3 and SH2 domains

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-ZZ-TEV-BICDR1

    Plasmid

    #111859

    Purpose

    Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, TEV site, and ZZ

    Expression

    Insect

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174642

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
    

    Tags

    SSPB(micro)-Venus

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Grb7-SH2

    Plasmid

    #46444

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb7 (GRB7 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 417-532

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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